HIV-1 Broadly Neutralizing Antibody Precursor B Cells Revealed by Germline-targeting Immunogen
Author | : |
Publisher | : |
Total Pages | : 6 |
Release | : 2016 |
ISBN-10 | : OCLC:982479774 |
ISBN-13 | : |
Rating | : 4/5 (74 Downloads) |
Download or read book HIV-1 Broadly Neutralizing Antibody Precursor B Cells Revealed by Germline-targeting Immunogen written by and published by . This book was released on 2016 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt: Induction of broadly neutralizing antibodies (bnAbs) is a major HIV vaccine goal. Germline-targeting immunogens aim to initiate bnAb induction by activating bnAb germline precursor B cells. Critical unmet challenges are to determine whether bnAb precursor naïve B cells bind germline-targeting immunogens and occur at sufficient frequency in humans for reliable vaccine responses. We employed deep mutational scanning and multi-target optimization to develop a germline-targeting immunogen (eOD-GT8) for diverse VRC01-class bnAbs. We then used the immunogen to isolate VRC01-class precursor naïve B cells from HIV-uninfected donors. Frequencies of true VRC01-class precursors, their structures, and their eOD-GT8 affinities support this immunogen as a candidate human vaccine prime. Lastly, these methods could be applied to germline targeting for other classes of HIV bnAbs and for Abs to other pathogens.